| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC | 124 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TCF8/ZEB1 Rabbit Polyclonal Antibody detects endogenous levels of TCF8/ZEB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ZEB1 also known as TCF8, ZFHX1A is a critical non-receptor transcription factor that plays a vital role in regulating gene expression during embryonic development and tissue maturation, particularly within mesodermal tissues. ZEB1 contains two zinc finger domains essential for DNA binding and transcriptional repression, as well as a homeodomain that facilitates protein-protein interactions. ZEB1′s three repression domains enable modulation of gene expression through site competition, enhancer silencing, and interaction with corepressors, making ZEB1 a key player in processes such as histogenesis and skeletal structure development. ZEB1′s interaction with the TSHβ gene T3-response element highlights ZEB1′s importance in regulating gene-specific responses to thyroid hormones, which are crucial for metabolic and developmental functions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SKOV3 cells, using TCF8/ZEB1 Rabbit Polyclonal Antibody (EAB14112) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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