| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC | 24 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | VHL Rabbit Polyclonal Antibody detects endogenous levels of VHL protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
VHL(Von Hippel-Lindau syndrome) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. VHL plays a crucial role as a tumor suppressor, and dysfunction is linked to von Hippel-Lindau syndrome, which predisposes individuals to several tumor types, including renal cell carcinoma, hemangioblastomas, and pheochromocytomas. VHL protein is primarily located in the cytoplasm and nucleus, where VHL interacts with the Elongin (SIII) complex, a heterotrimeric protein complex essential for regulating gene transcription. By binding to the Elongin B and C subunits, VHL protein inhibits the transcriptional activity of this complex, thereby playing a vital role in cellular processes such as oxygen sensing and the regulation of hypoxia-inducible factors. Different isoforms of VHL protein, resulting from alternatively spliced transcript variants, highlight the complexity of regulation and function, with variations in molecular weight observed across species. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Mouse brain cells, using VHL Rabbit Polyclonal Antibody (EAB14094) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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