| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC | 60 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Tyrosinase Rabbit Polyclonal Antibody detects endogenous levels of Tyrosinase protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Tyrosinase (TYR) is a type I membrane protein and copper-containing enzyme that plays a crucial role in melanin production, the primary pigment in vertebrates. Melanin biogenesis protects skin and eyes from UV radiation, and Tyrosinase catalyzes the critical and rate-limiting step of tyrosine hydroxylation in melanin biosynthesis. Deficiencies in Tyrosinase activity can lead to various forms of albinism, highlighting its importance in pigmentation. Tyrosinase interacts with related proteins TRP1 and TRP2, which are also expressed in melanocytes and contribute to melanin synthesis within melanosomes. These proteins share a similar transmembrane structure, featuring two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse liver cells, using Tyrosinase Rabbit Polyclonal Antibody (EAB14081) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 60 kDa; Observed band size: 80 kDa |
