| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 44 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TSG101 Rabbit Polyclonal Antibody detects endogenous levels of TSG101 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TSG101(Tumor susceptibility 101) is a multi-domain cytoplasmic protein and fundamental component of ESCRT complex I, which is intimately involved in regulating the trafficking of proteins throughout the endosomal compartment. TSG101 plays a crucial role in cellular processes as TSG101 is involved in the regulation of the endosomal sorting complex required for transport (ESCRT), which is essential for the degradation of membrane proteins and the regulation of cellular signaling pathways. This function is particularly important in tumor suppression, as proper functioning of the ESCRT machinery can prevent the accumulation of signaling molecules that promote cell proliferation and survival, thereby inhibiting tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raw264.7, Mouse liver cells, using TSG101 Rabbit Polyclonal Antibody (EAB14108) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human liver cancer tissue, using TSG101 Rabbit Polyclonal Antibody (EAB14108) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using TSG101 Rabbit Polyclonal Antibody (EAB14108)(green) at 1:200 dilution.
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