| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 81 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-SP1 (Thr453) Rabbit Polyclonal Antibody detects endogenous levels of SP1 protein only when phosphorylated at Thr453. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SP1(Specificity protein 1) is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. SP1 plays a crucial role in gene expression, cell growth, differentiation, and apoptosis. Initially identified as a factor that activates transcription from the SV40 promoter by binding to multiple GC boxes in the 21-base pair repeats, SP1′s DNA-binding specificity is conferred by three Cys2His2-type zinc finger motifs. Phosphorylation of SP1 can modulate activity and DNA-binding affinity, affecting the transcription of target genes. Located predominantly in the nucleus, SP1′s localization is essential for function as a transcriptional regulator. Dysregulation of SP1 has been implicated in various diseases, including cancer, highlighting importance in maintaining normal cellular functions. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549 cells, using Phospho-SP1 (Thr453) Rabbit Polyclonal Antibody (EAB14019) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 81 kDa; Observed band size: 90 kDa |
