| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 72 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:150-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Syk Rabbit Monoclonal Antibody detects endogenous levels of Syk protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains, positioning Syk as a crucial member of the protein kinase superfamily. Syk plays a vital role in B cell signaling by acting as a positive effector of B cell antigen receptor (CD79)-stimulated responses, facilitating calcium ion movement through two distinct phospho-regulated pathways. When unphosphorylated, calcium ions traverse a phosphoinositide 3-kinase-dependent pathway, while phosphorylation at tyrosine residues 348 and 352 shifts calcium flow to a phospholipase C gamma-dependent pathway. This regulatory mechanism is essential for various cellular processes, including differentiation, phagocytosis, proliferation, and B cell development. Syk expression is upregulated in T cell lymphoma, indicating potential involvement in tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1, SW620 cells, using Syk Rabbit Monoclonal Antibody (EAB22636) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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