| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 143 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SETDB1/ESET Rabbit Polyclonal Antibody detects endogenous levels of SETDB1/ESET protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SETDB1 also designated ESET, KMT1E is a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. SETDB1 plays a significant role in regulating chromatin structure. This highly conserved protein, consisting of 150 amino acids, methylates Lys-9 of histone H3, a modification serving as an epigenetic marker for transcriptional repression. Such methylation recruits heterochromatin protein 1 (HP1) to methylated histones, influencing gene expression and maintaining genomic stability. SETDB1 shows predominant expression in testis, with SETDB1 gene located on chromosome 1q21, and mutations can lead to loss of methyltransferase activity, underscoring SETDB1′s critical role in cellular function and development. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, Jurkat, 293T cells, using SETDB1/ESET Rabbit Polyclonal Antibody (EAB13978) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 143 kDa; Observed band size: 210 kDa |
