| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 39 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RASSF1 Rabbit Polyclonal Antibody detects endogenous levels of RASSF1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RASSF1 is a protein similar to the RAS effector proteins. RASSF1 plays a crucial role in cellular signaling pathways, particularly in the regulation of cell death and tumor suppression. Located at the human lung tumor suppressor locus 3p21.3, RASSF1 gene produces two major alternative transcripts, RASSF1A and RASSF1C, which interact with activated Ras proteins in a GTP-dependent manner. This interaction suggests RASSF1 may act as a Ras effector, mediating the apoptotic effects of oncogenic Ras, thereby influencing cell fate decisions such as senescence and apoptosis. Notably, RASSF1A is often epigenetically inactivated in various cancers, including lung and breast cancers, through hypermethylation of its promoter region, leading to a loss of its tumor suppressor function. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T cells, using RASSF1 Rabbit Polyclonal Antibody (EAB13949) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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