| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 154 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Rad50 Rabbit Monoclonal Antibody detects endogenous levels of Rad50 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Rad50 is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. Rad50 plays a crucial role in DNA double-strand break repair (DSBR) pathway, which is essential for maintaining genomic stability and preventing cell death, mutations, or neoplastic transformation. Rad50 functions as part of a protein complex that recognizes and binds to DNA breaks, facilitating recruitment of other repair proteins such as Rad51 and MRE11. This interaction is vital for homologous recombination repair process, which ensures accurate repair of DNA damage. Rad50′s ability to form a stable complex with Rad51 and BRCA1/BRCA2 highlights its importance in cellular response to DNA damage, as mutations in these pathways can lead to genomic instability and cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, PC12 cells, using Rad50 Rabbit Monoclonal Antibody (EAB21914) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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