| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC | 125 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | POLD1 Rabbit Polyclonal Antibody detects endogenous levels of POLD1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
POLD1 also known as DNA pol δ, DNA polymerase delta 1 is composed of two main subunits, p125 and p50, with p125 directly interacting with the sliding DNA clamp protein PCNA, which enhances processivity during DNA synthesis. POLD1 plays a crucial role in DNA replication, recombination, and repair, which are essential processes for maintaining genome stability. POLD1 is particularly important for the synthesis of the lagging strand during DNA replication, where DNA pol δ processes Okazaki fragments, ensuring that the newly synthesized DNA is accurate and complete. POLD1 is involved in the repair of damaged DNA, excising erroneous or damaged segments to maintain genomic integrity. POLD1 is subject to regulation by various cell cycle proteins, highlighting POLD1′s importance in coordinating DNA synthesis with the cell cycle. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SW480 cells, using POLD1 Rabbit Polyclonal Antibody (EAB13794) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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