| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 85 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IF/ICC=1:100-500 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Ezh2 Mouse Monoclonal Antibody detects endogenous levels of Ezh2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ezh2 also known as ENX-1, KMT6 is a member of the Polycomb group (PcG) gene family. Ezh2 plays a crucial role in regulating gene expression during embryonic development by repressing homeotic loci, which is essential for proper body plan formation. Ezh2 is predominantly expressed during early embryogenesis and later becomes localized to the central and peripheral nervous systems, as well as sites of fetal hematopoiesis, highlighting Ezh2′s importance in both developmental and adult tissue contexts. Ezh2 interacts with the proto-oncogene product Vav, suggesting involvement in the proliferation of both normal and malignant hematopoietic cells. Alterations in the regulation of target genes by Ezh2 may contribute to specific phenotypes associated with Down syndrome, underscoring Ezh2′s significance in both health and disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Jurkat, NIH/3T3 cells, using Ezh2 Mouse Monoclonal Antibody (EAB21969) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 85 kDa; Observed band size: 78-110 kDa |
