| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 69 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DDX5 Rabbit Polyclonal Antibody detects endogenous levels of DDX5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DDX5 also known as HUMP68 is a member of the DEAD box family of RNA helicases. DDX5 plays a crucial role in RNA metabolism, exhibiting RNA-dependent ATPase activity essential for unwinding RNA structures during processes such as transcription and translation. This activity facilitates proper processing of pre-mRNA and regulation of gene expression. DDX5 participates in organ differentiation during development and is expressed in early neural development, highlighting significance in embryonic growth and tissue formation. DDX5 localizes to the nucleus under normal conditions, interacting with transcriptional coactivators such as CBP and p300, further emphasizing a role in gene regulation. Phosphorylation by protein kinase C can inhibit DDX5 activity, indicating post-translational modifications significantly influence function. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using DDX5 Rabbit Polyclonal Antibody (EAB14732) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunofluorescence analysis of Hela cell, using DDX5 Rabbit Polyclonal Antibody (EAB14732) at 1:100 dilution.
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