| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 184 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | COL5A1 Rabbit Polyclonal Antibody detects endogenous levels of COL5A1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
COL5A1 also known as Collagen Type V Alpha 1 Chain plays a crucial role in the formation of the extracellular matrix, particularly in connective tissues such as tendons, ligaments, and skin. COL5A1 is essential for maintaining the structural integrity and tensile strength of these tissues, as COL5A1 contributes to the formation of collagen fibrils that provide support and elasticity. COL5A1 interacts with other collagen types, such as Type I and Type III collagens, to form a stable and functional extracellular matrix. This interaction is vital for normal tissue development and repair, and dysregulation of COL5A1 expression has been implicated in various connective tissue disorders, highlighting COL5A1 significance in both health and disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HuvEc, HepG2 cells, using COL5A1 Rabbit Polyclonal Antibody (EAB13768) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 184 kDa; Observed band size: 200 kDa |
