| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 14 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | p14 ARF Rabbit Polyclonal Antibody detects endogenous levels of p14 ARF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p14 ARF is encoded by the CDKN2A gene, which also produces p16 protein, and interacts with MDM2, a negative regulator of the p53 tumor suppressor pathway. By binding to MDM2, p14 ARF stabilizes p53 levels, leading to increased expression of p21 and subsequent cell cycle arrest in both G1 and G2/M phases. This regulatory mechanism is essential for preventing tumorigenesis, as damaged cells do not continue to divide. p14 ARF plays a crucial role in regulating the cell cycle by acting as a tumor suppressor, which is vital for maintaining cellular integrity and preventing uncontrolled cell proliferation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using p14 ARF Rabbit Polyclonal Antibody (EAB13750) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human placenta tissue, using p14 ARF Rabbit Polyclonal Antibody (EAB13750) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using p14ARF/CDKN2A Rabbit Polyclonal Antibody (EAB13750) at 1:100 dilution.
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