| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 59 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cdc25A Rabbit Polyclonal Antibody detects endogenous levels of Cdc25A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CDC25A is a member of the CDC25 family of phosphatases. Cdc25A plays a crucial role in cell cycle regulation, particularly in the transition from G2 phase to mitosis, by acting as a phosphatase that dephosphorylates and activates the cyclin-dependent kinase Cdc2. This activation is essential for the proper progression of mitosis, as it allows the cell to enter the mitotic phase and ensures accurate chromosome segregation. Dysregulation of Cdc25A can lead to uncontrolled cell division and has been implicated in various cancers, making Cdc25A a significant target for therapeutic intervention. Cdc25A is primarily located in the nucleus, where Cdc25A interacts with other cell cycle regulators, and Cdc25A activity is tightly controlled by phosphorylation and dephosphorylation events. |
| Product Image Gallery | |
 Western blot analysis of extracts on A2780, HepG2 cells, using Cdc25A Rabbit Polyclonal Antibody (EAB11576) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
