| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB | 60 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CARD8 Rabbit Polyclonal Antibody detects endogenous levels of CARD8 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CARD8 also known as DACAR, NDPP1, TUCAN, CARDINA is a member of the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 plays a crucial role in regulating cellular responses mediated by NF-κB activation. Such regulation is vital for maintaining cellular homeostasis and modulating inflammatory responses, making CARD8 an important player in apoptosis and chronic inflammatory disorders. CARD8 interacts with caspase-1, serving as a negative regulator of its activity, which is essential for controlling inflammatory processes. CARD8 has been shown to interact with IKKγ and FHL-2, further highlighting CARD8′s role in complex signaling pathways that influence cell survival and inflammation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Raji, K562 cells, using CARD8 Rabbit Polyclonal Antibody (EAB13717) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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