| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 187 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Complement C3 Rabbit Polyclonal Antibody detects endogenous levels of Complement C3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Complement C3 also known as CPAMD1, ASP, C3 is a pivotal protein in the complement system of the innate immune response. Synthesized primarily in the liver, C3 is secreted into the bloodstream and resides mainly in the extracellular space, which is crucial for its role in immediate defense against pathogens. The extracellular location allows C3 to interact directly with foreign invaders, leading to their opsonization and the activation of the complement cascade. Upon activation, C3 is cleaved into C3a and C3b fragments; C3a acts as an anaphylatoxin mediating inflammatory responses, while C3b binds to pathogen surfaces to enhance phagocytosis by immune cells. Dysregulation of C3 can result in increased susceptibility to infections or autoimmune disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on HL60, HepG2 cells, using Complement C3 Rabbit Polyclonal Antibody (EAB13714) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 187 kDa; Observed band size: 120, 180 kDa |
