| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 208 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BRCA1 Rabbit Polyclonal Antibody detects endogenous levels of BRCA1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BRCA1 also known as RNF53 is a 190 kD nuclear phosphoprotein that plays a crucial role in maintaining genomic stability and is essential for the repair of DNA double-strand breaks through homologous recombination, a process vital for preventing mutations that can lead to cancer. BRCA1 is predominantly located in the nucleus, where BRCA1 interacts with other proteins involved in DNA repair and cell cycle regulation, underscoring BRCA1′s importance in tumor suppression. Mutations in the BRCA1 gene can disrupt these critical functions, leading to an increased risk of breast and ovarian cancers, making BRCA1 research essential for understanding cancer biology and developing targeted therapies. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293, MCF7, A549 cells, using BRCA1 Rabbit Polyclonal Antibody (EAB10614) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 208 kDa; Observed band size: 220 kDa  Immunohistochemistry of paraffin-embedded Human brain tissue, using BRCA1 Rabbit Polyclonal Antibody (EAB10614) at 1:100 dilution.
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