| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 79 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bcl-6 Rabbit Monoclonal Antibody detects endogenous levels of Bcl-6 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bcl-6 is a critical transcriptional repressor involved in regulating gene expression by binding to Stat recognition-like DNA elements. Bcl-6 plays a pivotal role in germinal center development and the differentiation of T helper cells into Th1 and Th2 subsets, essential processes in the adaptive immune response. Post-translational modifications of Bcl-6, such as acetylation, phosphorylation, and ubiquitination, are crucial for regulating stability, localization, and transcriptional activity. HDAC- and SIR2-dependent acetylation of Bcl-6 modulates activity by inhibiting the recruitment of histone deacetylase complexes, thereby downregulating repressive functions. These modifications are vital for the fine-tuning of Bcl-6′s role in various cellular processes, including the negative regulation of NFκB expression, which is important for controlling inflammatory responses and maintaining immune system balance. |
| Product Image Gallery | |
 Western blot analysis of extracts on Daudi, Ramos, Rat spleen cells, using Bcl-6 Rabbit Monoclonal Antibody (EAB21912) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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