| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 43 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-EDG1 (Thr236) Rabbit Polyclonal Antibody detects endogenous levels of EDG1 protein only when phosphorylated at Thr236. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
EDG1 also known as S1P1, S1PR1 is a member of the endothelial differentiation gene (EDG) family of G protein-coupled receptors. EDG1 play a crucial role in mediating the effects of lysophospholipid signaling molecules such as sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA). EDG1 is primarily located on the cell surface and is involved in various cellular processes, including cell survival, growth, migration, and differentiation, by coupling to Gi proteins to activate downstream signaling pathways like Akt. This is particularly important in glioma cells, where EDG1 expression is associated with tumor progression and survival. EDG1′s ability to interact with multiple G proteins influences a wide range of physiological responses, making EDG1 a significant target for research in cancer biology and therapeutic development. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells treated with UV, using Phospho-EDG1 (Thr236) Rabbit Polyclonal Antibody (EAB13609) at 1:500 dilution. The lane on the left is blocked with the phospho peptide.
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