| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC | 12 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | S100A4 Rabbit Polyclonal Antibody detects endogenous levels of S100A4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
S100A4 also known as MTS1 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100A4 plays a crucial role in tumor progression and metastasis, as well as significantly stimulating angiogenesis. S100A4 is primarily located in the cytoplasm and nucleus of various cell types, where S100A4 interacts with multiple signaling pathways that regulate cell growth and survival. S100A4′s presence in these cellular compartments is vital, as S100A4 modulates key processes such as apoptosis and cell migration, which are essential for cancer development and progression. Notably, S100A4 has been shown to cooperate with the tumor suppressor protein p53, binding to its C-terminal regulatory domain and inhibiting its DNA-binding activity, thereby enhancing p53-dependent apoptosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SKOV3 cells, using S100A4 Rabbit Polyclonal Antibody (EAB13578) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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