| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC | 25 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Rab27A Rabbit Polyclonal Antibody detects endogenous levels of Rab27A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
The Rab family of low molecular weight GTPases are critical regulators of vesicular transport. Rab proteins cycle between an active GTP-bound state, which recruits specific effector proteins, and an inactive GDP-bound state. Two members of this family, Rab27a and Rab27b, have overlapping functions, but differ in tissue specificity. Rab27a is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Rab27b is also expressed in pituitary tissue, but is more significantly expressed in brain and spleen. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, U937 cells, using Rab27A Rabbit Polyclonal Antibody (EAB22398) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue, using Rab27A Rabbit Polyclonal Antibody (EAB22398) at 1:100 dilution.
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