| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 98 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PML Rabbit Polyclonal Antibody detects endogenous levels of PML protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PML also known as TRIM19 is a member of the tripartite motif (TRIM) family. PML is a zinc finger transcription factor and is crucial for regulating gene expression involved in cell differentiation and apoptosis. PML′s role in these processes is particularly significant in acute promyelocytic leukemia, where PML gene undergoes a chromosomal translocation with the retinoic acid receptor α (RARα) gene, resulting in the formation of the PML/RARα chimeric protein. This aberrant protein disrupts normal myeloid differentiation by recruiting the nuclear co-repressor-histone deacetylase complex, which is essential for retinoic acid-dependent differentiation, and also induces gene hypermethylation, leading to the silencing of critical genes and promoting leukemogenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T, A549, 22RV1 cells, using PML Rabbit Polyclonal Antibody (EAB13570) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue, using PML Rabbit Polyclonal Antibody (EAB13570) at 1:100 dilution.
 Immunofluorescence analysis of A549 cell, using PML Rabbit Polyclonal Antibody (EAB13570) at 1:100 dilution.
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