| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 273 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cleaved-Notch1 (Val1754) Rabbit Polyclonal Antibody detects endogenous levels of fragment of activated Notch1 protein resulting from cleavage adjacent to Val1754. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Notch1 is a member of the NOTCH family of proteins. Notch1 plays a pivotal role in cell fate determination during embryonic and adult development. Upon binding to its ligands, such as Jagged1, Jagged2, and Delta-like proteins, Notch1 undergoes proteolytic cleavage, releasing its intracellular domain, which translocates to the nucleus to regulate gene expression. This signaling pathway is crucial for processes like differentiation, proliferation, and apoptosis, impacting the development of various tissues and organs. Dysregulation of Notch1 has been implicated in several cancers, including T-cell acute lymphoblastic leukemia, highlighting its importance in maintaining cellular homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, HepG2, Mouse brain cells, using Cleaved-Notch1 (Val1754) Rabbit Polyclonal Antibody (EAB13565) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 273 kDa; Observed band size: 110 kDa |
