| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 78 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MMP-9 Mouse Monoclonal Antibody detects endogenous levels of MMP9 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MMP-9(Matrix metalloproteinase 9) is a member of the matrix metalloproteinase (MMP) family. MMP-9 plays a critical role in the degradation of extracellular matrix components like collagen, fibronectin, and laminin, which is vital for processes like tissue remodeling and inflammation. MMP-9 interacts with several key proteins that modulate its function, including tissue inhibitors of metalloproteinases (TIMPs), particularly TIMP-1, which binds to the catalytic domain of MMP-9, inhibiting its proteolytic activity. MMP-9 also works in conjunction with MMP-2 in the breakdown of type IV collagen in the basement membrane, contributing to processes like angiogenesis and tumor metastasis. Additionally, MMP-9 interacts with integrins and CD44, which can facilitate its localization to the cell surface and regulate cell migration. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Siha cells, using MMP-9 Mouse Monoclonal Antibody (EAB21407) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 78 kDa; Observed band size: 92 kDa  Immunohistochemistry analysis of paraffin-embedded Human brain tissues, using MMP-9 Mouse Monoclonal Antibody (EAB21407) at 1:200 dilution.
 Immunofluorescence analysis of NIH/3T3 cell, using MMP-9 Mouse Monoclonal Antibody (EAB21407) at 1:200 dilution.
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