www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 50 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PDK1 (Tyr243) Rabbit Polyclonal Antibody detects endogenous PDK1 protein only when phosphorylated at Tyr243. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PDK1(pyruvate dehydrogenase kinase 1) plays a crucial role in cellular metabolism by regulating the activity of the mitochondrial pyruvate dehydrogenase complex, which is essential for converting pyruvate into acetyl-CoA, a key substrate for the citric acid cycle. This regulation is vital for maintaining energy homeostasis, particularly in response to varying nutrient availability. The activity of PDK1 is influenced by several factors, including the levels of ATP and NADH, which signal the energy status of the cell. PDK1 undergoes post-translational modifications, such as phosphorylation, which can modulate activity and stability, thereby impacting metabolic pathways. |
