| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 12 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MIF Rabbit Polyclonal Antibody detects endogenous levels of MIF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MIF also known as MMIF is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a crucial role in modulating macrophage and T cell functions, making MIF an essential regulator of host immune response to infection. MIF is primarily produced by the pituitary gland and is expressed in various cell types, including monocytes, macrophages, and differentiating immunological cells in the eye lens and brain, as well as fibroblasts. MIF is found at sites of inflammation, indicating MIF′s significant role in regulating macrophage activity during host defense. Elevated levels of MIF have been associated with severe sepsis and septic shock, where MIF enhances the production of other inflammatory cytokines such as tumor necrosis factor α, interleukin-1, and interferon-γ, thereby promoting a systemic inflammatory response. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, PC12 cells, using MIF Rabbit Polyclonal Antibody (EAB13553) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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