| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 45 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody detects endogenous levels of p47phox only when phosphorylated at Ser359. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p47phox also known as NOXO2, SH3PXD1A, NCF1 is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. p47phox plays a crucial role in the immune response by participating in the activation of the NADPH oxidase complex, which is essential for the production of reactive oxygen species in neutrophils and other phagocytic cells. This process is vital for the destruction of pathogens, and mutations in p47phox have been linked to chronic granulomatous disease, a hereditary condition that impairs the immune system′s ability to fight infections. p47phox structure includes two Src homology 3 (SH3) domains, which are important for interaction with p67-phox, another key component of the NADPH oxidase complex. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with Nocodazole, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using Phospho-p47phox (Ser359) Rabbit Polyclonal Antibody (EAB13495) at 1:200 dilution.
|
