| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF/ICC | 50 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:100-500 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CA9 Mouse Monoclonal Antibody detects endogenous levels of CA9 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CA IX also known as CA9 is a member of the carbonic anhydrase family that plays a crucial role in regulating pH and facilitating the transport of carbon dioxide and bicarbonate across cell membranes, which is vital for maintaining acid-base balance in tissues. CA IX protein is particularly important in tumor biology, as CA IX expression increases in hypoxic conditions commonly found in solid tumors, such as clear-cell renal carcinomas. CA IX expression patterns serve as potential biomarkers for cancer diagnosis and prognosis while highlighting a role in tumor progression and adaptation to the tumor microenvironment. Understanding CA IX function and regulation can provide insights into therapeutic strategies targeting tumor metabolism and hypoxia. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T cells, using CA IX/CA9 Mouse Monoclonal Antibody (EAB13487) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 50 kDa; Observed band size: 38-48 kDa  Immunohistochemical analysis of paraffin-embedded Human lung caricnoma tissue, using CA IX/CA9 Mouse Monoclonal Antibody (EAB13487) at 1:200 dilution.
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