| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 25 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HMGB1 Rabbit Polyclonal Antibody detects endogenous levels of HMGB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HMGB1(High mobility group box 1) also known as HMG1 is a protein that belongs to the High Mobility Group-box superfamily. HMGB1 play a crucial role in the regulation of gene expression and chromatin structure. Specifically, HMGB1 interacts with various transcription factors, such as Oct-2 and members of the NFκB family, enhancing their ability to activate transcription. This interaction is vital for cellular responses to stress and inflammation, as HMGB1 can act as a signaling molecule that influences the immune response. HMGB1 is predominantly located in the nucleus, where binding to DNA induces structural changes that facilitate the accessibility of transcription machinery to specific genes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, K562 cells, using HMGB1 Rabbit Polyclonal Antibody (EAB13469) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 25 kDa; Observed band size: 30 kDa  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue, using HMGB1 Rabbit Polyclonal Antibody (EAB13469) at 1:100 dilution.
|
