| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 183 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Dnmt1 Rabbit Polyclonal Antibody detects endogenous levels of Dnmt1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Dnmt1 is an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. Dnmt1 is primarily located in the nucleus, where Dnmt1 interacts with hemimethylated DNA to ensure faithful transmission of epigenetic information during DNA replication. The ability of Dnmt1 to recognize and bind to hemimethylated sites is vital for preserving methylation status of genes, thereby influencing developmental processes and cellular identity. Dysregulation of Dnmt1 has been implicated in various diseases, including cancer, making Dnmt1 a significant target for research into epigenetic therapies. |
| Product Image Gallery | |
 Western blot analysis of extracts on HL60, Rat ovaries cells, using Dnmt1 Rabbit Polyclonal Antibody (EAB11653) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
