| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 58 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PKM2 Rabbit Polyclonal Antibody detects endogenous levels of PKM2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKM2 is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 plays a crucial role in cellular metabolism, specifically in glycolysis, where PKM2 catalyzes the conversion of phosphoenolpyruvate to pyruvate, generating adenosine triphosphate (ATP) in the process. This function is vital for energy production in cells, particularly in tissues with high metabolic demands, such as muscle and brain. PKM gene encodes two isoforms, M1 and M2, through alternative splicing, with both isoforms forming tetramers that are activated by fructose 1,6-bisphosphate. Notably, M2-type isoform has been shown to interact with Oct-4, a key transcription factor in stem cell biology, enhancing transcriptional activity and thereby influencing cellular differentiation and proliferation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HT29 cells, using PKM2 Rabbit Polyclonal Antibody (EAB13390) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human testis tissue, using PKM2 Rabbit Polyclonal Antibody (EAB13390) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon tissue, using PKM2 Rabbit Polyclonal Antibody (EAB13390) at 1:200 dilution.
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