| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 48 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody detects endogenous levels of Smad3 when phosphorylated at Ser425. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Smad3 also known as LDS3 and MADH3 is a critical intracellular mediator in the Transforming Growth Factor-beta (TGF-β) signaling pathway. Smad3 playing a pivotal role in regulating gene expression related to cell proliferation, differentiation, apoptosis, and extracellular matrix production. By transducing signals from the cell membrane to the nucleus, Smad3 facilitates the transcription of target genes that are essential for maintaining cellular homeostasis and tissue development. Dysregulation of Smad3 activity is implicated in various pathological conditions, including fibrosis, cancer progression, and immune response modulation, making Smad3 a significant target for research and therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells treated with IGF-I, using Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody (EAB13333) at 1:500 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 48 kDa; Observed band size: 58 kDa  Western blot analysis of extracts on L929, Mouse brain, Rat kidney cells, using Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody (EAB13333) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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