| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 91, 90 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Stat5 (Ser726/731) Rabbit Polyclonal Antibody detects endogenous levels of Stat5 when phosphorylated at Ser726/731. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Stat5 also known as stat5a/b, stat5a/stat5b is a member of the STAT family of transcription factors. Stat5 proteins play a pivotal role in mediating cellular responses to cytokines and growth factors by undergoing phosphorylation through receptor tyrosine kinases or G protein-coupled receptors. Upon activation, stat5a and stat5b form homo- or heterodimers that translocate to the nucleus to initiate transcription of genes involved in cell proliferation, differentiation, and apoptosis, particularly within lymphohematopoietic cells. This signaling is crucial for processes like T-cell receptor signaling, mammary gland development, and regulation of liver gene expression related to sexual dimorphism. Aberrant stat5 activity is associated with various diseases, including acute promyelocytic leukemia, where stat5b can fuse with the retinoic acid receptor alpha gene. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with EGF, using Phospho-Stat5 (Ser726/731) Rabbit Polyclonal Antibody (EAB13314) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
|
