| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 77 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Gab1 (Tyr659) Rabbit Polyclonal Antibody detects endogenous levels of Gab1 protein only when phosphorylated at Tyr659. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Gab1 is a member of the IRS1-like multisubstrate docking protein family. Gab1 plays a crucial role in cellular signaling pathways, particularly in growth factor receptor activation. Gab1 functions as an adapter protein that facilitates signal transmission from activated receptor tyrosine kinases to downstream signaling molecules, influencing cellular responses such as proliferation and differentiation. Gab1 is essential in epidermal growth factor receptor (EGFR) and insulin receptor signaling pathways, where Gab1 forms complexes with other adapter proteins like GRB2 and Sos, linking these receptors to the Ras signaling pathway. This interaction regulates various cellular processes, including metabolism and cell growth, making Gab1 crucial for maintaining cellular homeostasis and responding to external growth signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells treated with Insulin, using Phospho-Gab1 (Tyr659) Rabbit Polyclonal Antibody (EAB13403) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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