| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 35 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-CREB (Ser142) Rabbit Polyclonal Antibody detects endogenous levels of CREB only when phosphorylated at Ser142. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CREB also known as CREB-1 is a crucial transcription factor that regulates gene expression by binding to the cAMP response element (CRE) in the promoters of target genes, thereby modulating essential cellular processes such as metabolism, neuronal plasticity, and survival. Upon phosphorylation at Serine 133 by protein kinase A, CREB-1 undergoes a conformational change that facilitates interaction with the CREB-binding protein (CBP), promoting the assembly of the basal transcription complex and activating transcriptional programs critical for cellular responses to hormonal and stress signals. This regulatory mechanism underscores CREB-1′s pivotal role in neoglucogenesis through interaction with the nuclear coactivator PGC-1, as well as involvement in the pathogenesis of type II diabetes and dilated cardiomyopathy, highlighting significance in both metabolic regulation and cardiovascular health. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with PMA, using Phospho-CREB (Ser142) Rabbit Polyclonal Antibody (EAB13269) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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