| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 62 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Cortactin (Tyr466) Rabbit Polyclonal Antibody detects endogenous levels of cortactin protein only when phosphorylated at Tyr466. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cortactin is characterized by distinct structure, featuring tandem 37 amino acid repeats at the N-terminus and an SH3 domain at the C-terminus, which are essential for binding and stabilizing filamentous actin (F-actin) as well as interacting with various signaling proteins. These structural domains facilitate Cortactin′s role in actin polymerization, cell motility, and formation of cellular protrusions such as lamellipodia and invadopodia, making Cortactin vital for processes like cell migration and invasion. Cortactin′s function is regulated through tyrosine phosphorylation by Src family kinases, which modulates actin-binding affinity and cross-linking activity, thereby influencing cytoskeletal dynamics and cellular structural integrity. This structural flexibility allows Cortactin to participate in diverse cellular functions, including endocytosis and signal transduction pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells treated with H2O2, using Phospho-Cortactin (Tyr466) Rabbit Polyclonal Antibody (EAB13261) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 62 kDa; Observed band size: 80 kDa |
