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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 89 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-KAP1/TIF1β (Ser824) Rabbit Polyclonal Antibody detects endogenous levels of KAP1/TIF1β protein only when phosphorylated on Ser824. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
KAP1 also known as TF1β, TRIM28 is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. KAP1 plays a crucial role in transcriptional regulation by interacting with the Kruppel-associated box (KRAB) repression domain found in various transcription factors. This interaction is vital for mediating transcriptional repression, which is essential for processes such as cellular differentiation and response to stress. The human KAP1 gene is located on chromosome 19q13.4 and encodes an 835 amino acid nuclear protein, underscoring its importance in gene expression regulation and cellular homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with LPS, using Phospho-KAP1/TIF1β (Ser824) Rabbit Polyclonal Antibody (EAB13203) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 89 kDa; Observed band size: 110 kDa |
