| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 20 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IL-10 Rabbit Polyclonal Antibody detects endogenous levels of IL-10 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IL-10(Interleukin-10) is a 178 amino acid protein primarily secreted by TH2 clones and plays a crucial role in immune regulation. One of its key functions is the suppression of pro-inflammatory cytokine production by TH1 cells, which is vital for maintaining immune homeostasis and preventing excessive inflammatory responses that can lead to tissue damage. Additionally, IL-10 inhibits the activation of macrophages and B lymphocytes by downregulating CD25, the IL-2 receptor, thereby modulating the immune response. The importance of IL-10 in immune regulation is underscored by elevated expression in various pathological conditions, including HIV-1 infection, where IL-10 has been implicated in disease progression. Human and murine IL-10 share significant sequence identity, with 81% similarity at the amino acid level, and both forms exist as non-covalently linked homodimers in solution, exerting biological effects through the IL-10 receptor, a glycoprotein that can be induced in macrop |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1, HepG2, Mouse liver cells, using IL-10 Rabbit Polyclonal Antibody (EAB13121) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, using IL-10 Rabbit Polyclonal Antibody (EAB13121) at 1:100 dilution.
 Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue, using IL-10 Rabbit Polyclonal Antibody (EAB13121) at 1:200 dilution.
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