| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 68 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PKCζ (Thr410) Rabbit Polyclonal Antibody detects endogenous levels of PKCζ only when phosphorylated at Thr410. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKCζ also known as PKC zeta, PRKCZ is an atypical member of the protein kinase C family, playing a crucial role in various cellular processes such as cell growth, differentiation, survival, gene expression, and membrane function. Unlike conventional PKCs, PKCζ activation is independent of calcium and diacylglycerol (DAG), allowing unique function within signaling pathways. PKCζ is involved in the regulation of NF-κB activation, insulin signaling, cell polarity, and the PI3K/Akt pathway, making PKCζ significant in understanding diseases like diabetes, cancer, and immune disorders. PKCζ′s involvement in cell survival and proliferation underscores PKCζ′s importance as a potential therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with PMA, using Phospho-PKCζ (Thr410) Rabbit Polyclonal Antibody (EAB13111) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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