| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 58 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PPARγ Rabbit Polyclonal Antibody detects endogenous levels of PPARγ protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PPARγ also known as PPARgamma, PPARG is a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARγ plays a crucial role in regulating adipocyte differentiation and lipid metabolism, making PPARγ essential for maintaining energy homeostasis and metabolic health. Located primarily in the nucleus, PPARγ functions as a transcription factor that forms heterodimers with retinoid X receptors (RXRs) to regulate the expression of genes involved in insulin sensitivity and inflammation. This regulation is particularly important in the context of metabolic disorders, as PPARγ is implicated in conditions such as obesity, type 2 diabetes, and cardiovascular diseases. Furthermore, PPARγ is known to interact with various ligands, including prostanoids and thiazolidinediones, which can activate transcriptional activity, thereby influencing adipocyte function and inflammatory responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc, Mouse skin cells, using PPARγ Rabbit Polyclonal Antibody (EAB10536) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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