| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, ChIP | 15 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 ChIP=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody detects endogenous levels of histone H3 protein only when acetylated at Lys27. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Histone H3 is one of the four core histones (H2A, H2B, H3, and H4) essential for the formation of nucleosomes, which are fundamental units of chromatin structure in eukaryotic cells. Histone H3 is a core part of the nucleosome′s octameric structure that wraps DNA, regulating gene expression and DNA replication. Histone H3 plays a crucial role in chromatin′s structural organization, essential for DNA packaging within the nucleus. Histone H3 maintains genomic stability and facilitates transcription and repair processes. Histone H3 undergoes various post-translational modifications, including methylation and acetylation, influencing DNA and protein interactions, affecting chromatin structure and function. These modifications regulate gene expression and impact cellular differentiation and environmental response. |
| Product Image Gallery | |
 Western blot analysis of extracts on C6 cells, using Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody (EAB23704) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 15 kDa; Observed band size: 15-17 kDa |
