| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 17 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | UBC13 Rabbit Monoclonal Antibody detects endogenous levels of UBC13 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
UBC13 also known as UBE2N, BLU is a crucial member of the E2 ubiquitin-conjugating enzyme family, consisting of 152 amino acids. UBC13 plays a vital role in the ubiquitination process, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBC13 is unique in forming a heterodimer with Mms2 (UBE2V2) to catalyze the synthesis of non-canonical polyubiquitin chains, a process distinct from the typical proteasomal degradation pathway. This unique function allows for the regulation of various cellular processes, including DNA repair, cell cycle progression, and cellular differentiation, contributing to cell survival following DNA damage. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, Jurkat cells, using UBC13 Rabbit Monoclonal Antibody (EAB22082) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
