| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 90 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | UHRF1 Mouse Monoclonal Antibody detects endogenous levels of UHRF1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
UHRF1 also known as Np95 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. UHRF1 is predominantly localized in the nucleus, where UHRF1 plays a vital role in cellular proliferation by functioning as an E3 ubiquitin-protein ligase. UHRF1 facilitates the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to target proteins, marking them for degradation, which is essential for maintaining cellular homeostasis and regulating the cell cycle. Additionally, UHRF1 directly interacts with Dnmt1, a maintenance DNA methyltransferase, ensuring stable association with chromatin, which is critical for proper regulation of gene expression and epigenetic inheritance. The overexpression of UHRF1 in cancer cells highlights UHRF1′s potential involvement in carcinogenesis, making UHRF1 a significant target for research in cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7 cells, using UHRF1 Mouse Monoclonal Antibody (EAB22037) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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