| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 25 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Thymidine Kinase Rabbit Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Thymidine Kinase also known as TK1 is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. TK1 plays a crucial role in DNA synthesis by catalyzing the phosphorylation of deoxythymidine to deoxythymidine 5′-phosphate, a key step in the nucleotide salvage pathway. This function is particularly important as TK1 facilitates the incorporation of deoxythymidine into DNA, which is essential for cell proliferation and repair. TK1 activity is significantly elevated in actively dividing cells, making TK1 a valuable biomarker for assessing cell proliferation in various clinical contexts, especially in the diagnosis and monitoring of hematological malignancies. Additionally, TK1 is critical for the efficacy of several antiviral drugs, such as azidothymidine (AZT), and is utilized in the selection of hybridoma cell lines during monoclonal antibody production. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205 cells, using Thymidine Kinase Rabbit Polyclonal Antibody (EAB11842) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Thymidine Kinase Rabbit Polyclonal Antibody (EAB11842) at 1:100 dilution.
 Immunofluorescence analysis of HepG2 cell, using Thymidine Kinase Rabbit Polyclonal Antibody (EAB11842) at 1:100 dilution.
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