| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IP, IF, ELISA | 84 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:100-500 IF=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TBK1/NAK Mouse Monoclonal Antibody detects endogenous levels of TBK1/NAK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TBK1 also known as NAK is primarily located in the cytoplasm, where TBK1 interacts with various signaling molecules to modulate NFκB activity. TBK1 plays a crucial role in regulating the NFκB signaling pathway, vital for immune response and inflammation. TBK1′s cytoplasmic localization enables rapid response to cellular stress and infection, facilitating IκB protein phosphorylation, which leads to IκB degradation and subsequent NFκB release and activation. This process is essential for transcribing genes involved in immune responses, making TBK1 a key player in defense mechanisms. TBK1 also interacts with TRAF2 and TANK, further highlighting TBK1′s role in NFκB activation pathway. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Jurkat, HepG2 cells, using TBK1/NAK Mouse Monoclonal Antibody (EAB21899) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
