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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 71 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TRIM25 Rabbit Polyclonal Antibody detects endogenous levels of TRIM25 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TRIM25 also known as Z147, RNF147, ZNF147, EFP is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The structural composition of EFP allows for precise interaction with estrogen receptors, facilitating transcriptional regulation of target genes in response to estrogen signaling. This interaction is vital for growth, differentiation, and function of various tissues, highlighting EFP′s importance in mediating estrogen′s effects. The regulation of EFP gene expression involves multiple promoter elements and interacting factors, which underscores EFP′s role in a broader signaling cascade that influences secondary estrogen-responsive genes. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Mouse brain, Rat brain cells, using TRIM25 Rabbit Polyclonal Antibody (EAB11860) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human breast cancer tissue, using TRIM25 Rabbit Polyclonal Antibody (EAB11860) at 1:100 dilution.
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