| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 38 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SAE1 Rabbit Polyclonal Antibody detects endogenous levels of SAE1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SAE1(SUMO1 activating enzyme subunit 1) also known as AOS1, UBL1E1 is a crucial component of the ubiquitin-like (UBL) conjugation pathway, specifically functioning as a ubiquitin-activating E1 ligase. SAE1 plays a vital role in the activation of UBL1, which is essential for various cellular processes, including cell cycle regulation and stress response mechanisms. SAE1 operates as a dimeric enzyme, facilitating the ATP-dependent activation of UBL1, and can form heterodimers with UBLE1A and UBLE1B, enhancing functional capacity. Proper functioning of SAE1 is critical, as disruptions in the UBL conjugation pathway can lead to various diseases, including cancer and neurodegenerative disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293, Rat brain cells, using SAE1 Rabbit Polyclonal Antibody (EAB11853) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tisse, using SAE1 Rabbit Polyclonal Antibody (EAB11853) at 1:200 dilution.
 Immunofluorescence analysis of HuvEc cell, using SAE1 Rabbit Polyclonal Antibody (EAB11853) at 1:100 dilution.
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