| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 181 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:50-200 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Brm Rabbit Monoclonal Antibody detects endogenous levels of Brm protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Brm also designated SMARCA2 is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Brm plays a crucial role in gene regulation. Brm functions as a transcriptional activator of Hox genes, interacting with nearly all transcriptionally active chromatin while maintaining a non-overlapping pattern with Polycomb, a known repressor of Hox gene transcription. This unique interaction is vital for proper expression of homeotic genes, as the Brm complex acts as an essential coactivator for the trithorax group protein Zeste, which binds to DNA and activates these genes. Notably, a reduction in Brm function significantly decreases the association of RNA polymerase II with Drosophila salivary gland chromosomes, indicating that chromatin remodeling activity of the Brm complex is fundamental for facilitating transcription by RNA polymerase II. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, U2OS cells, using Brm Rabbit Monoclonal Antibody (EAB22282) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 181 kDa; Observed band size: 200 kDa |
