| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 82 | All |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SV40 Large T Antigen Rabbit Monoclonal Antibody detects endogenous levels of SV40 Large T Antigen protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SV40 Large T antigen plays a crucial role in the viral life cycle and is essential for the transformation of host cells, as SV40 large T antigen interacts with key cellular proteins. Notably, SV40 large T antigen binds to the retinoblastoma (Rb) tumor suppressor protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the release of E2F, promoting cell cycle progression and contributing to oncogenic transformation. Additionally, SV40 large T antigen′s ability to inactivate the p53 tumor suppressor gene further underscores SV40 large T antigen′s importance in cellular transformation and cancer research. |
| Product Image Gallery | |
 Western blot analysis of extracts on different cells, using SV40 Large T Antigen Rabbit Monoclonal Antibody (EAB22302) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: HL60 Lane2: A549 |
